WebApr 15, 2007 · We applied this technique, ChR2-assisted circuit mapping (CRACM), to map long-range callosal projections from layer (L) 2/3 of the somatosensory cortex. WebMay 8, 2007 · Regulatory elements from the mouse thy1.2 gene (Fig. 1A) were used to drive neuron-specific expression of ChR2-YFP.Differences in the position and/or copy numbers of transgene insertion produced patterns of expression in subsets of neurons that varied among the different mouse lines but were consistent within a particular line ().From our …
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WebSNP Association Results in European-Ancestry Familiesa Allele 1 Total Counts Allele 2 Total Counts SNP LOC A1 Frq T NT A2 Frq T NT rs12210050 chr6:420489 T 0.23 565 459 C 0.77 1763 1869 rs12426725 chr12:80367259 A 0.15 308 411 G 0.85 2030 1927 rs1170612 chr2:124699526 T 0.22 593 481 C 0.78 1745 1857 rs16934812 … WebNov 6, 2014 · Blue light (5 s pulse; 470±20 nm; ~0.5 mW/mm 2) and yellow light (5 s pulse; 575±25 nm; 2 mW/mm 2) was delivered from an Arc lamp (EXFO) by a 10x objective (Zeiss M2Bio) to the head of a forward-moving worm to activate ChR2 and NpHR to trigger reversals, respectively. We scored it as a positive response if the animal stopped forward … knotfest lineup brisbane
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WebUse the table and filters below to find ready-to-use AAV preparations of optogenetics plasmids available in Addgene's collection. Excitatory. Wild-type ChR2. ChR2/H134R. … WebApr 2, 2010 · As the authors coexpressed ChR2 and eNpHR3.0 in the same neuron, they found that blue-light stimulation of ChR2 also weakly activated eNpHR3.0, and vice versa. This resulted in a 40% decrease in peak currents for both excitation and inhibition when compared to cells expressing either molecule individually. Nevertheless, eNpHR3.0 is a … WebJul 25, 2014 · The most widely used optogenetic tool, Channelrhodopsin2 (ChR2), is both light- and voltage-sensitive. A light-triggered action potential or light-driven perturbations of ongoing electrical ... knotfest melbourne times