Dgtp triphosphohydrolase inhibitor
WebOct 31, 2024 · The dNTP triphosphohydrolase SAMHD1 is a regulator of cellular dNTP pools. Given its central role in nucleotide metabolism, SAMHD1 performs important … WebApr 20, 2016 · The bacteriophage T7 encoded inhibitor (gp1.2) of E. coli dGTP triphosphohydrolase. Released: 20 Apr 2016. DOI: 10.2210/pdb2mdp/pdb. Source organism: Escherichia phage T7. Entry authors: Mueller GA, London RE Function and Biology Details. ... Inhibitor of dGTPase Chain: A.
Dgtp triphosphohydrolase inhibitor
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WebJan 23, 2007 · Disclaimer Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional … WebIn 2001, Kicska et al. developed immucillin H, now better known as forodesine, as a small molecule PNP inhibitor (PNP-I). 4 The intention of the authors was to kill lymphocytes by elevating intracellular dG and consequently dGTP levels and to thereby eliminate leukemic cells. Forodesine was subsequently tested in clinical trials and was found ...
WebJan 22, 2024 · dNTP triphosphohydrolase (TPH) belongs to the histidine/aspartate (HD) superfamily and catalyzes the hydrolysis of dNTPs into 2′-deoxyribonucleoside and inorganic triphosphate. TPHs are required for cellular dNTP homeostasis and DNA replication fidelity and are employed as a host defense mechanism. PA1124 from the pathogenic … WebSep 25, 1988 · In this report we show that the gene 1.2 protein is a specific inhibitor of the E. coli dGTPase. Gene 1.2 protein inhibits dGTPase activity by forming a complex with the dGTPase with an apparent stoichiometry of two monomers of gene 1.2 protein/tetramer of dGTPase. The interaction is reversible with a half-life of the complex of 30 min and an ...
WebApr 21, 2014 · The degradative dNTP triphosphohydrolase activity of the sterile α-motif/histidine-aspartate domain-containing protein 1 (SAMHD1) enzyme helps maintain optimal dNTP balances for DNA replication and also serves as an HIV-1 restriction factor in resting CD4 + target cells of HIV by depleting dNTP substrates of reverse transcriptase. … WebDeoxyguanosinetriphosphate triphosphohydrolase (dGTPase) is a triphosphohydrolase that has a preference for dGTP over other dNTPs, they hydrolase dGTP to form deoxyguanosine and triphosphate. Escherichia coli dGTPase (Dgt) has been shown to have a strong single-stranded DNA-binding (ssDNA) activity [PMID:25694425] which is …
WebFeb 8, 2024 · Compared to the genome of the related phage, Hafnia phage Ca genome contains extra genes including DNA mimic ocr, dGTP triphosphohydrolase inhibitor, …
WebSep 25, 1993 · The dgt gene of Escherichia coli encodes a deoxyguanosine triphosphate triphosphohydrolase (dGTPase) that hydrolyzes dGTP to deoxyguanosine and tripolyphosphate. The enzyme is highly specific for dGTP which is hydrolyzed with a Km of 2-5 microM. Nitrocellulose filter binding assays demonstrate that, … gardner transportation indianaWebSep 25, 1988 · Both the binding of the substrate dGTP and of the inhibitor gene 1.2 protein induce conformational changes in dGTPase. The conformation of the enzyme in the … black panther 1 verThe dNTPase activity of SAMHD1 is stimulated by dGTP24,25. dGTP, but not the other dNTPs, is also required for the formation/maintenance of the SAMHD1 tetramer (Fig. 1). A detailed view of the dGTP-binding site provides an explanation for the stabilization of the SAMHD1c′ tetramer by dGTP. Two dGTP … See more The SAM domain and its amino-terminal region are not required for tetramer formation or the dNTPase activity of SAMHD1 (refs 27, 28). However, SAMHD1c (120–626) … See more We have co-crystallized SAMHD1c′ with its dGTP/dATP substrates and solved the structure by the molecular replacement method, using 3U1N … See more To determine the importance for tetramer formation of dGTP/dATP binding in the allosteric site, we tested several SAMHD1 mutants. These … See more The substrate-binding pocket in the active site is formed by amino acids in the major lobe (Fig. 5b). Extensive interactions between the substrate dGTP and surrounding amino … See more gardnertown united methodist churchWebMay 7, 2024 · However, the mechanism behind dGTP selectivity is unclear. Here we present the free-, ligand (dGTP)- and inhibitor (GTP)-bound structures of hexameric Ec-dGTPase, including an X-ray free-electron laser structure of the free EcEc-dGTPase and SAMHD1 share similar active-site and HD motif architectures; however, Ec black panther #1 valueWebOct 1, 1988 · In this report we show that the gene 1.2 protein is a specific inhibitor of the E. coli dGTPase. Gene 1.2 protein inhibits dGTPase activity by forming a complex with the … gardnertrucking.comWebing of the substrate dGTP and of the inhibitor gene 1.2 protein induce conformational changes in dGTPase. The conformation of the enzyme in the presence of ... triphosphate … gardner trucking accidentWebSep 6, 2024 · Escherichia coli encodes a 2′-deoxyguanosine-5′-triphosphate (dGTP) triphosphohydrolase (dGTPase; Dgt), encoded by the dgt gene, which catalyzes the … gardner trucking human resources