Psin-ef2 vector
WebApr 27, 2024 · The DAB2IP expression construct was generated by subcloning PCR-amplified full-length human DAB2IP complementary DNA into the pSin-EF2-puro lentiviral vector (Addgene, beijing, China) by EcoRI and NheI. pSin-EF2-puro-DAB2IP or the empty lentiviral vector and 2 packaging plasmids pM2.G and psPAX2 (Addgene, beijing, China) … WebJan 8, 2024 · The pSin-EF2-puro-ARNTL, pSin-EF2-puro-vector and psin-EF2-puro-CDK5 plasmids were obtained from Land. Hua Gene Biosciences (Guangzhou, China), and the psin-EF2-puro-vector plasmid served as a control. To generate stable cell lines, lentivirus packing expression plasmids were co-transfected into 293 T cells for 48 h. Then, SUNE1 …
Psin-ef2 vector
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WebJul 6, 2024 · Although pSIN-EF2-(Pou4f3 or GFI1)-Pur and pLV-ATOH1-IRES-eGFP belong to the third-generation of lentiviral transfer vectors [36,37], pSIN-EF2-Pou4f3-Pur and pSIN … WebThe pSin-EF2- GFP-Puro (EF1 a promoter) provided maximal GFP expression in both the undifferentiated cells as well as the day 10 differentiated cells with greater than 95% of …
WebPsin Ef2 Puromycin Lentiviral Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article … WebpSin (Search Vector Database) Vector type Synthetic Biology Growth in Bacteria Bacterial Resistance (s) Ampicillin, 100 μg/mL Growth Temperature 37°C Growth Strain (s) NEB Stable Copy number Low Copy Gene/Insert Gene/Insert name Sindbis Glycoproteins Cloning Information Cloning method Unknown Resource Information Supplemental Documents
WebThe empty pSin-EF2 vector (lenti-vector) was used as a control. Observation of morphologic changes. The cells were seeded into six-well culture (Corning Incorporated, Corning, NY, USA) plates and transferred with miR-16 or miR-Ctrl. After incubation for 24 hours, the cellular morphology was observed by phase contrast microscope (Olympus America ... WebApr 6, 2024 · Briefly, the pSin-EF2-TIPE3-HA or empty vector, as well as the lentivirus packaging plasmids psPAX2 and pMD2.G, were co-transfected into 293FT cells using the calcium phosphate method. After...
WebMay 11, 2024 · abstract type AbstractPolynomial end """ Polynomial <: AbstractPolynomial Polynomials written in the canonical form --- Polynomial(v::T) where T<:Union{Vector{<:Real}, NTuple{<:Any, <:Real}}) Construct a `Polynomial` from the list of the coefficients. The coefficients are assumed to go from power 0 in the ascending order.
WebNov 17, 2024 · GFP-human Med15 and TagRFP-human DYRK3 fusion protein were subcloned into the lentiviral expression vector pSin-EF2 by ClonExpress II One Step Cloning Kit (Vazyme Biotech, C112). Primers used to clone lentiviral vectors are provided in Additional file 6: Table S3. The human embryonic kidney 293T cell line was used as a host … compound bicep exerciseWebSystemic delivery of pSin-EF2-ELMO1-Pur facilitates continuous ELMO1 expression in colonic tissues. [A] Immunohistochemical staining of ELMO1 in the colons of untreated … echo air conditionerWebJul 18, 2024 · The full length of CTBP1-DT was generated by subcloning the PCR-amplified human CTBP1-DT sequence and cloned into pSin-EF2-vector. The DDUP ORF with FLAG-tag expression plasmid was generated by cloning the full length of CTBP1-DT. echo airdyneWebFeb 3, 2024 · The AKR1B10 CDS (coding DNA sequence) was cloned into the lentiviral expression vector pSIN-EF2-Puro (hereinafter referred to as pSIN-EF2) to construct the lentiviral vector pSIN-EF2/AKR1B10. The method to establish stable AKR1B10-expression cell line has been described previously 23. AKR1B10-shRNA stable transfection using … echo alias python python3 /.bashrcWebJan 7, 2024 · product information Catalog Number : 16579 Product Name : pSin-EF2-Oct4-Pur article : bacterial resistance : Ampicillin cloning : growth notes : The psPAX2 … echo alfordWebFeb 3, 2024 · Lentiviral vector construction, virus infection and the establishment of stable cell line. The AKR1B10 CDS (coding DNA sequence) was cloned into the lentiviral … echo alarm not going offWebOct 19, 2024 · The pSin-EF2-puro-ZNF671-HA or pSin-EF2-puro-vector plasmids were obtained from Land. Hua Gene Biosciences (Guangzhou, China); pSin-EF2-puro-Vector … compound biscuits