WebbThe PCR products of 100 to 1000 base pair were purified using the purification kit method (WizPrepTM Gel/PCR Purification Mini Kit) as instructed. Briefly, PCR products were treated with a PCR purification buffer (PCR purification buffer: PCR sample = 5:1). The mixture was transferred to the spin column and centrifuged at 14000 rpm for 1 minute. WebbThe construct comprises a 49-bp 5′ UTR, including the T7 promoter (T7) and ribosome binding site (RBS), followed by the gene of interest (ATG start codon and the codon-optimized USCTX sequence) and a 35-bp 3′ UTR, including a TAA stop codon and T7 terminator (Ter).
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WebbDownload the product protocol from Thermo Fisher Scientific for PureLink™ Quick Plasmid Miniprep Kit below. Download manufacturer protocol Videos Check out videos that might be relevant for performing Plasmid Isolation Aeromonas spp. using PureLink™ Quick Plasmid Miniprep Kit from Thermo Fisher Scientific. WebbThermo Scientific GeneJET Genomic DNA Purification Kit is developed for fast and efficient purification of high product genomic DNA from various mammalian cell culture … how to make your home off grid
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WebbPlasmid DNA obtained with peqGOLD Plasmid Miniprep kits can directly be used for a wide range of downstream applications. Suitable for the simultaneous processing of multiple samples Short hands-on and preparation times (<15 minutes) No need for organic extractions or precipitations Complete enzymatic degradation of all cellular RNA WebbEach run included 94 samples, the positive control included in the kit, and a negative control. For quantitative PCR, quality control, and normalization purposes, the Bio-Rad CFX96 Real-Time PCR Detection System (Bio-Rad Laboratories, Inc., Hercules, CA, USA) was utilized. 2.4. Sequencing WebbThermo Scientific GeneJET Plasmid Miniprep Kit K0502, K0503 Wash the column Add 500 μL of Wash Solution and centrifuge for 30-60 s. Discard the flow-through. Centrifuge … mug smartphoto